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We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method.gene trap mutations are reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector.pFloxin. Floxin technology is applicable to the existing collection of 24.49 compatible gene trap cell lines.which should enable the high-throughput modification of many genes in mouse ESCs.
Floxin may be taken with or without food.but take it at the same time each day.
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